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sendai virus sev strain cantell  (ATCC)


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    ATCC sendai virus sev strain cantell
    Sendai Virus Sev Strain Cantell, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sendai+virus+sev+strain+cantell/Sendai+virus%3B+Strain%3A+Cantell/pm41405988-586-0-8
    Average 94 stars, based on 46 article reviews
    sendai virus sev strain cantell - by Bioz Stars, 2026-09
    94/100 stars

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    Virus:

    Article Title: Z Proteins of New World Arenaviruses Bind RIG-I and Interfere with Type I Interferon Induction
    Article Snippet: 293A and A549 cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (Invitrogen, Carlsbad, CA) and 100 μg/ml penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO). .. Sendai virus (SeV) strain Cantell (VR907) was purchased from the ATCC (Manassas, VA). ..

    Article Title: Influenza A virus modulates ACE2 expression and SARS-CoV-2 infectivity in human cardiomyocytes
    Article Snippet: Influenza virus strain A/Victoria/361/2011 (H3N2) and Influenza virus strain B/Texas/06/2011 (Yamagata), NR-44024 were purchased from BEI Resources propagated in specific pathogen free 10-day embryonated chicken eggs. .. Sendai virus (SeV) strain Cantell, VR-907 was purchased from ATCC, propagated in specific pathogen free 10-day embryonated chicken eggs. ..

    Article Title: Palmitoylation on Conserved and Nonconserved Cysteines of Murine IFITM1 Regulates Its Stability and Anti-Influenza A Virus Activity
    Article Snippet: .. We also performed a specificity control using Sendai virus (SeV) strain Cantell (ATCC). ..

    Article Title: Z Proteins of New World Arenaviruses Bind RIG-I and Interfere with Type I Interferon Induction
    Article Snippet: 293A and A549 cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (Invitrogen, Carlsbad, CA) and 100 g/ml penicillin-streptomycin (Sigma-Aldrich, St. Louis, MO). .. Sendai virus (SeV) strain Cantell (VR907) was purchased from the ATCC (Manassas, VA). ..

    Article Title: Palmitoylation on Conserved and Nonconserved Cysteines of Murine IFITM1 Regulates Its Stability and Anti-Influenza A Virus Activity
    Article Snippet: .. We also performed a specificity control using Sendai virus (SeV) strain Cantell (ATCC). ..

    Article Title: Influenza A virus modulates ACE2 expression and SARS-CoV-2 infectivity in human cardiomyocytes
    Article Snippet: Influenza virus strain B/Texas/06/2011 (Yamagata) , BEI Resources , Cat# NR-44024. .. Sendai virus (SeV) strain Cantell , ATCC , Cat# VR-907. .. Human metapneumovirus (MPV), strain NL/1/00 (A1) , Generated by Dr. Jianrong Li (Ohio State University) , Dr. Li, Ohio State University .

    Article Title: A 20-amino-acid Cardiovirus protein exhibits cytokine-mimicry activity to regulate viral replication.
    Article Snippet: .. Sendai virus (SeV) strain Cantell was obtained through ATCC (VR-907), propagated in chicken embryonated eggs, and virus titer was determined using plaque assay. ..

    Article Title: Systematic Discovery of TLR Signaling Components Delineates Viral-Sensing Circuits
    Article Snippet: .. Sendai virus (SeV) strain Cantell and Encephalomyocarditis virus (EMCV) strain EMC (ATCC), Newcastle disease virus (NDV) strain Hitchner B1 (gift from A. Garcia-Sastre), and vesicular stomatitis virus (VSV) strain Indiana (U. von Andrian), were used for infections. ..

    Control:

    Article Title: Palmitoylation on Conserved and Nonconserved Cysteines of Murine IFITM1 Regulates Its Stability and Anti-Influenza A Virus Activity
    Article Snippet: .. We also performed a specificity control using Sendai virus (SeV) strain Cantell (ATCC). ..

    Article Title: Palmitoylation on Conserved and Nonconserved Cysteines of Murine IFITM1 Regulates Its Stability and Anti-Influenza A Virus Activity
    Article Snippet: .. We also performed a specificity control using Sendai virus (SeV) strain Cantell (ATCC). ..

    Plaque Assay:

    Article Title: A 20-amino-acid Cardiovirus protein exhibits cytokine-mimicry activity to regulate viral replication.
    Article Snippet: .. Sendai virus (SeV) strain Cantell was obtained through ATCC (VR-907), propagated in chicken embryonated eggs, and virus titer was determined using plaque assay. ..



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    sendai virus sev strain cantell  (ATCC)
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    In A) A549 cells were infected with RSV A2 (MOI=3; 8 hrs) before analysis of WCE by immunoblotting using an anti-RSV NS1/NS2 antibody (left panel). The specificity of the anti-RSV NS1/NS2 antibody was confirmed by transfection of siRNA-targeting NS1 or NS2 before infection with RSV A2 (right panel). Anti-actin was used as a loading control. B) A549 cells were transiently transfected with an empty vector or the hNS2-pcDNA5 plasmid containing a humanized version of the RSV Long NS2 coding sequence and analyzed by immunoblotting using anti-RSV NS1/NS2 antibodies. C) A549 cells were co-transfected with an IFNBprom-pGL3 firefly luciferase plasmid, a pRL-null renilla luciferase control plasmid (pRL-Null, Promega) and increasing amounts of hNS2-pcDNA5 plasmid before infection with <t>Sendai</t> virus (SeV) at 80HAU/10 6 cells. Luciferase activities were expressed as fold activation over the corresponding NS condition after normalization with renilla luciferase activities. Data (mean ±SEM; n=3) were analyzed using a two-tailed paired Student’s t-test.
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    In A) A549 cells were infected with RSV A2 (MOI=3; 8 hrs) before analysis of WCE by immunoblotting using an anti-RSV NS1/NS2 antibody (left panel). The specificity of the anti-RSV NS1/NS2 antibody was confirmed by transfection of siRNA-targeting NS1 or NS2 before infection with RSV A2 (right panel). Anti-actin was used as a loading control. B) A549 cells were transiently transfected with an empty vector or the hNS2-pcDNA5 plasmid containing a humanized version of the RSV Long NS2 coding sequence and analyzed by immunoblotting using anti-RSV NS1/NS2 antibodies. C) A549 cells were co-transfected with an IFNBprom-pGL3 firefly luciferase plasmid, a pRL-null renilla luciferase control plasmid (pRL-Null, Promega) and increasing amounts of hNS2-pcDNA5 plasmid before infection with <t>Sendai</t> virus (SeV) at 80HAU/10 6 cells. Luciferase activities were expressed as fold activation over the corresponding NS condition after normalization with renilla luciferase activities. Data (mean ±SEM; n=3) were analyzed using a two-tailed paired Student’s t-test.
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    In A) A549 cells were infected with RSV A2 (MOI=3; 8 hrs) before analysis of WCE by immunoblotting using an anti-RSV NS1/NS2 antibody (left panel). The specificity of the anti-RSV NS1/NS2 antibody was confirmed by transfection of siRNA-targeting NS1 or NS2 before infection with RSV A2 (right panel). Anti-actin was used as a loading control. B) A549 cells were transiently transfected with an empty vector or the hNS2-pcDNA5 plasmid containing a humanized version of the RSV Long NS2 coding sequence and analyzed by immunoblotting using anti-RSV NS1/NS2 antibodies. C) A549 cells were co-transfected with an IFNBprom-pGL3 firefly luciferase plasmid, a pRL-null renilla luciferase control plasmid (pRL-Null, Promega) and increasing amounts of hNS2-pcDNA5 plasmid before infection with <t>Sendai</t> virus (SeV) at 80HAU/10 6 cells. Luciferase activities were expressed as fold activation over the corresponding NS condition after normalization with renilla luciferase activities. Data (mean ±SEM; n=3) were analyzed using a two-tailed paired Student’s t-test.
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    murine sendai virus (sev) cantell strain  (Charles River Laboratories)
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    In A) A549 cells were infected with RSV A2 (MOI=3; 8 hrs) before analysis of WCE by immunoblotting using an anti-RSV NS1/NS2 antibody (left panel). The specificity of the anti-RSV NS1/NS2 antibody was confirmed by transfection of siRNA-targeting NS1 or NS2 before infection with RSV A2 (right panel). Anti-actin was used as a loading control. B) A549 cells were transiently transfected with an empty vector or the hNS2-pcDNA5 plasmid containing a humanized version of the RSV Long NS2 coding sequence and analyzed by immunoblotting using anti-RSV NS1/NS2 antibodies. C) A549 cells were co-transfected with an IFNBprom-pGL3 firefly luciferase plasmid, a pRL-null renilla luciferase control plasmid (pRL-Null, Promega) and increasing amounts of hNS2-pcDNA5 plasmid before infection with <t>Sendai</t> virus (SeV) at 80HAU/10 6 cells. Luciferase activities were expressed as fold activation over the corresponding NS condition after normalization with renilla luciferase activities. Data (mean ±SEM; n=3) were analyzed using a two-tailed paired Student’s t-test.
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    sendai virus (sev, cantell strain  (Charles River Laboratories)
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    In A) A549 cells were infected with RSV A2 (MOI=3; 8 hrs) before analysis of WCE by immunoblotting using an anti-RSV NS1/NS2 antibody (left panel). The specificity of the anti-RSV NS1/NS2 antibody was confirmed by transfection of siRNA-targeting NS1 or NS2 before infection with RSV A2 (right panel). Anti-actin was used as a loading control. B) A549 cells were transiently transfected with an empty vector or the hNS2-pcDNA5 plasmid containing a humanized version of the RSV Long NS2 coding sequence and analyzed by immunoblotting using anti-RSV NS1/NS2 antibodies. C) A549 cells were co-transfected with an IFNBprom-pGL3 firefly luciferase plasmid, a pRL-null renilla luciferase control plasmid (pRL-Null, Promega) and increasing amounts of hNS2-pcDNA5 plasmid before infection with <t>Sendai</t> virus (SeV) at 80HAU/10 6 cells. Luciferase activities were expressed as fold activation over the corresponding NS condition after normalization with renilla luciferase activities. Data (mean ±SEM; n=3) were analyzed using a two-tailed paired Student’s t-test.
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    In A) A549 cells were infected with RSV A2 (MOI=3; 8 hrs) before analysis of WCE by immunoblotting using an anti-RSV NS1/NS2 antibody (left panel). The specificity of the anti-RSV NS1/NS2 antibody was confirmed by transfection of siRNA-targeting NS1 or NS2 before infection with RSV A2 (right panel). Anti-actin was used as a loading control. B) A549 cells were transiently transfected with an empty vector or the hNS2-pcDNA5 plasmid containing a humanized version of the RSV Long NS2 coding sequence and analyzed by immunoblotting using anti-RSV NS1/NS2 antibodies. C) A549 cells were co-transfected with an IFNBprom-pGL3 firefly luciferase plasmid, a pRL-null renilla luciferase control plasmid (pRL-Null, Promega) and increasing amounts of hNS2-pcDNA5 plasmid before infection with Sendai virus (SeV) at 80HAU/10 6 cells. Luciferase activities were expressed as fold activation over the corresponding NS condition after normalization with renilla luciferase activities. Data (mean ±SEM; n=3) were analyzed using a two-tailed paired Student’s t-test.

    Journal: bioRxiv

    Article Title: A Stable Reporter Cell Line to Study Respiratory Syncytial Virus NS2-Mediated Inhibition of IFNB Promoter Activation

    doi: 10.64898/2026.02.24.706909

    Figure Lengend Snippet: In A) A549 cells were infected with RSV A2 (MOI=3; 8 hrs) before analysis of WCE by immunoblotting using an anti-RSV NS1/NS2 antibody (left panel). The specificity of the anti-RSV NS1/NS2 antibody was confirmed by transfection of siRNA-targeting NS1 or NS2 before infection with RSV A2 (right panel). Anti-actin was used as a loading control. B) A549 cells were transiently transfected with an empty vector or the hNS2-pcDNA5 plasmid containing a humanized version of the RSV Long NS2 coding sequence and analyzed by immunoblotting using anti-RSV NS1/NS2 antibodies. C) A549 cells were co-transfected with an IFNBprom-pGL3 firefly luciferase plasmid, a pRL-null renilla luciferase control plasmid (pRL-Null, Promega) and increasing amounts of hNS2-pcDNA5 plasmid before infection with Sendai virus (SeV) at 80HAU/10 6 cells. Luciferase activities were expressed as fold activation over the corresponding NS condition after normalization with renilla luciferase activities. Data (mean ±SEM; n=3) were analyzed using a two-tailed paired Student’s t-test.

    Article Snippet: Alternatively, subconfluent monolayers of cells were infected with Sendai virus (SeV) Cantell strain (Charles River Laboratories) at 40 hemagglutinin units (HAU)/10 6 cells in the minimum volume of serum free medium.

    Techniques: Infection, Western Blot, Transfection, Control, Plasmid Preparation, Sequencing, Luciferase, Virus, Activation Assay, Two Tailed Test

    A) A549 cells transfected with either 3xFlag-pCMV-3Tag1a control or hNS2-3xFlag-pCMV-3Tag1a plasmids, followed by antibiotic selection to establish stable population and isolate control monoclonal cell lines (A549-ctrl) and NS2-expressing monoclonal cell lines (A549-Flag-NS2). The expression of Flag-tagged NS2 in the resulting monoclonal cells was confirmed by immunoblotting using anti-Flag antibody. Anti-actin was used as a loading control. Transiently transfected cells were used as positive control of Flag-NS2 expression. B) Pools of four A549-Ctrl or of A549-Flag-NS2 monoclonal cells were infected with Sendai virus (SeV) for the indicated times. IFNβ, TNF and SeV N transcript levels were quantified by RT-qPCR. C) A549-Ctrl and A549-Flag-NS2 cells were stimulated with recombinant human IFNβ for the indicated times. WCE were analyzed by immunoblotting using antibodies against total STAT2, phosphorylated STAT2 (Tyr689). Anti-actin was used as a loading control.

    Journal: bioRxiv

    Article Title: A Stable Reporter Cell Line to Study Respiratory Syncytial Virus NS2-Mediated Inhibition of IFNB Promoter Activation

    doi: 10.64898/2026.02.24.706909

    Figure Lengend Snippet: A) A549 cells transfected with either 3xFlag-pCMV-3Tag1a control or hNS2-3xFlag-pCMV-3Tag1a plasmids, followed by antibiotic selection to establish stable population and isolate control monoclonal cell lines (A549-ctrl) and NS2-expressing monoclonal cell lines (A549-Flag-NS2). The expression of Flag-tagged NS2 in the resulting monoclonal cells was confirmed by immunoblotting using anti-Flag antibody. Anti-actin was used as a loading control. Transiently transfected cells were used as positive control of Flag-NS2 expression. B) Pools of four A549-Ctrl or of A549-Flag-NS2 monoclonal cells were infected with Sendai virus (SeV) for the indicated times. IFNβ, TNF and SeV N transcript levels were quantified by RT-qPCR. C) A549-Ctrl and A549-Flag-NS2 cells were stimulated with recombinant human IFNβ for the indicated times. WCE were analyzed by immunoblotting using antibodies against total STAT2, phosphorylated STAT2 (Tyr689). Anti-actin was used as a loading control.

    Article Snippet: Alternatively, subconfluent monolayers of cells were infected with Sendai virus (SeV) Cantell strain (Charles River Laboratories) at 40 hemagglutinin units (HAU)/10 6 cells in the minimum volume of serum free medium.

    Techniques: Transfection, Control, Selection, Expressing, Western Blot, Positive Control, Infection, Virus, Quantitative RT-PCR, Recombinant